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KMID : 0350519960490010297
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Journal of Catholic Medical College
1996 Volume.49 No. 1 p.297 ~ p.307
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The Effects of Epidermal Growth Factor on Survival of Fat Tissue Autograft in the Rabbit
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Abstract
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Autotransplantation of the fat tissue has been used for restoration of facial contour, and reconstruction of facial atrophy, depression scars, and other surgical defects. However, the survival rate of the autotransplanted fat tissue has been
quoted
from
30% to 80% of its initial volume.
Illouz and de Villern(1989) reported that some hormones and growth factors including insulin, estrogen, and adipocyte growth factor control the proliferation of the fat tissue. In 1985, Wong and Ng reported that bo9th epidermal growth factor and
nerve
growth factor significantly stimulated lipogenesis from glucose in isolated rat adipocyte. Shillabber et al (1989) proposed the possibility of the differentiation of the preadipochyte to the mature adipocyte by cell-specific growth factor. This
study
was designed to investigate the efficacy of epidermal growth factor (EGF) to increase the viability of the autotransplanted fat tissue. Using 16 New Zealand white rabbits, the groin fat pads were harvested and grafted to the 2×2cm sized skin
pockets
which were prepared at the ventral surfaces of the ears. In the EGF treated group, an EGF gel(50㎍/g) was applied inside of the pocket at the time of operation, and an EGF ointement (50㎍/g) was appolied to the skin surface of the pocket once a
day
for
5 day for 5 days. In the control group, an EGF free gel and ointment were applied as a same method. Survival of the fat graft was analysed and documented with measurements of the changes in its volume, weight, thickness 30 days postoperatively,
and,
histologic findings 3, 10, 30 days postoperatively.
@ES The results were as follows:
@EN 1) On the gross observation, the EGF treated grafts showed less contracture and atrophy than the control ones.
2) The weight, volume, and thickness of the EGF treated fat grafts were increased significantly (P<0.05) compared to those of the control ones 30 days postoperatively.
3) In the histologic examination, inflammatory cells infiltration was observed in both control and EGF treated grafts 3 days postoperatively. The vascularity increased in the EGF treated grafts 10 days postoperatively. On 30 days
postoperatively,
the
control grafts showed fat cell destruction with microcystic change and the EGF treated grafts showed abundant collagen fiber deposit between well preserved adipocytes.
With the above results, epidermal growth factor is likely to enhance survival of the autotransplanted fat tissue in the rabbit ear.
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KEYWORD
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